Signal Transduction Reagents and Kits
Promega NIK Kinase Enzyme System, 10microg
Recombinant human NIK (325-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NIK is a mitogen-activated protein kinase kinase kinase 14 (MAP3K14), which binds to TRAF2 and stimulates NF-kappaB activity.
Promega ADP-Glo Kinase Assay + Aurora B Kinase Enzyme System, 1 each
Recombinant full-length human Aurora B was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. Aurora B is a member of the Aurora kinase family that associates with microtubules during chromosome movement and segregation.
Promega p70S6Kb Kinase Enzyme System, 10microg
Recombinant full-length human p70S6Kb was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p70S6Kb is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases and is activated by mitogenic stimuli.
Promega ADP-Glo™ Kinase Assay + MLCK Kinase Enzyme System
Recombinant human mLCK (1425-1776) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag. mLCK or myosin light chain kinase is a muscle member of the immunoglobulin gene superfamily and a calcium/calmodulin-dependent protein kinase.
Promega ABL1 (T315I) Kinase Enzyme System, 1mg
Recombinant human ABL1 (T315I) (27-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega CDK6/CyclinD3 Kinase Enzyme System, 1mg
Recombinant full-length human CDK6 and CyclinD3 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.
Promega Kinase-Glo™ Luminescent Kinase Assay
The Kinase-Glo™ Luminescent Kinase Assays are homogeneous non-radioactive systems for determining the activity of purified kinases by quantifying the amount of ATP remaining in solution following a kinase reaction.
Promega MINK1 Kinase Enzyme System, 10microg
Recombinant human MINK1 (1-320) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. MINK1 is a member of the germinal center family of kinases that are homologous to the Ste20 family and regulate a wide variety of cellular processes.
Promega ADP-Glo Kinase Assay + AKT2 Kinase Enzyme System, 1 each
Recombinant full-length human AKT2 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. AKT2 or Protein Kinase B beta (PKB beta) is a serine/threonine kinase that is a member of the AKT family.
Promega MAPKAPK5 Kinase Enzyme System, 1mg
Recombinant full-length human MAPKAPK5 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MAPKAPK5 is a member of the serine/threonine kinase family that responds to cellular stress and proinflammatory cytokines.
Promega ACK Kinase Enzyme System, 10microg
ACK is a tyrosine kinase that binds CDC42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of CDC42Hs.
Enzo Life Sciences SAG, (1mg), CAS Number: 364590-63-6
Smoothened ligand. A potent cell-permeable benzothiophene compound that modulates the coupling of Smo with its downstream effector by interacting with the Smo heptahelical domain (KD = 59 nM). Shown to induce Hedgehog pathway activation (EC50= ~3 nM in NIH 3T3-derived Shh-LIGHT2 cells) and counteracts Cyclopamine (BML-GR334) inhibition of Smo. Reported to act as an activator at low concentrations and as an inhibitor at very high concentrations. Formula: C28H28ClN3OS. MW: 490.1. CAS Number: 364590-63-6. Purity: ≥95% (HR-MS, 1H-NMR, 13C-NMR). Appearance: White to off-white solid. Solubility: Soluble in DMSO (10mg/ml), sparingly soluble in water (acidification with HCl increases water solubility). Use/Stability: Stable for at least 6 months when stored at -20°C. Long Term Storage: -20°C
Aat Bioquest Calbryte™ 520 AM | 1mg
Calbryte™ 520, AM is a novel fluorescent and cell-permeable indicator for the measurement of intracellular calcium. Like other dye AM esters, Calbryte™ 520 AM is non-fluorescent and non-activatable. Once Calbryte™ 520 AM enters the cell, it is readily hydrolyzed by intracellular esterase where it becomes activated and responsive to calcium. The activated indicator is now a polar molecule that is incapable of freely diffusing through the cell membrane, essentially trapping it inside the cell. Upon binding calcium ions, Calbryte™ 520 produces a bright fluorescence signal with extremely high signal/background ratio. It has the identical excitation and emission wavelength as Fluo-4, thus the same Fluo-4 assay settings can be readily applied to Calbryte™ 520-based calcium assays.